NAD+

Description

NAD+ Reference Material | Nicotinamide Adenine Dinucleotide

NAD+ (MW 663.43 g/mol) is a dinucleotide coenzyme involved in over 500 enzymatic reactions across cellular metabolism. As a research reference material, NAD+ serves as a redox substrate in metabolic enzyme assays and as a co-substrate for NAD+-consuming signaling enzymes including sirtuins (SIRT1–7), poly(ADP-ribose) polymerases (PARPs), and CD38 NAD+ hydrolase.

Research Applications

• NAD+/NADH cycling assays in mitochondrial function studies
• Sirtuin (SIRT1–7) deacetylase kinetics — Km/Vmax determinations
• PARP1/PARP2 enzyme activity and DNA damage response assays
• CD38 NAD+ hydrolase and cyclic ADP-ribose (cADPR) production studies
• Cellular NAD+ quantification (enzymatic cycling, HPLC, fluorometric methods)
• Wallerian degeneration and axonal NAD+ research models
• Neuronal viability assays and neurodegeneration model systems

NAD+ vs. Precursors in Research

For studies requiring acute, direct NAD+ delivery to cells and tissues, research-grade NAD+ provides immediate substrate availability compared to precursor compounds (NMN, NR) that require intracellular enzymatic conversion steps — an important distinction for kinetic and time-course experimental designs.

Specifications

• Form: Lyophilized NAD+ disodium salt
• Molecular Weight: 663.43 g/mol
• Purity: ≥99% (HPLC verified)
• Storage: -20°C long-term; 2–8°C working stock; light and moisture sensitive

For laboratory research use only. Not for human or veterinary use.